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#UFC503024) as follows: First, ultrafiltration was conducted with 500 µL of TCST-EDTA (20 mM Tris-HCl (pH 7.5) buffer containing 1 mM DTT and 0.005% Triton X-100) in the presence of 300 mM NaCl four times, then it was conducted with 500 µL of TCST-EDTA four times, to remove NaCl